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Molecular docking and dynamics simulation predicted the binding energy and stability of these constituents to UA related transporters

Luciferase reporter assays A bicistronic vector pcDNA3 RLuc POLIRES FLuc (empty reporter) (Addgene, 45642) and pcDNA3 RLuc POLIRES FLuc containing human PRKAA1 5UTR upstream of FLuc (5UTR reporter) were transfected into shCtrl and shGDH1 or shYBX1 Huh7 cells using Lipofectamine 2000 (Thermo Fisher)

22 Lac3 was corrected by the signal intensity of the resonance at t = 0 min to avoid contamination from lipids by subtracting the natural abundant signal at around 2.4 ppm

44 ClinicalTrials.gov (2021c)

Bernstein JL

Eur J Pharm Biopharm 163:6071 Tavanai E, Mohammadkhani G (2017) Role of antioxidants in prevention of age-related hearing loss: a review of literature