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Activity of enzyme was determined in 100 mM potassium phosphate buffer (pH 6.5) with 1 mM l-chloro-2,4-dinitrobenzene (CDNB) and 4 mM GSH as substrates by following the change in absorbance at 340 nm ( 9.6 mM 1

Accurate in situ monitoring of mitochondrial H 2 O 2 by robust SERS nanoprobes with a AuSe interface

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Study limitations and future directions Despite the inherent amplification bias associated with the long-range PCR, which can result in variable read depth across different amplicons, our assay achieved complete coverage of all target regions and reliably detected clinically relevant variants