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The membranes were incubated with the following primary antibodies: GGCT (ab198503, 1:500, Abcam, Cambridge, UK), xCT (ab238078, 1:500, Abcam), -actin (M177-3, 1:2,000, MBL, Tokyo, Japan), ACSL4 (F6T3Z, #38493, 1:1000, Cell Signaling Technology, Danvers, MA, USA), LPCAT3 (EPR28798-172, 1:1000, Abcam), and GPX4 (ab125066, 1:1000, Abcam), followed by secondary antibodies