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For 200 ml of culture, Expi293F cells at about 3 million per ml cell density were transfected with 95 g of the ZSWIM8 expression plasmid, 47.5 g of the ELOB expression plasmid, 47.5 g of the ELOC expression plasmid, 10 g of pMaxGFP and 600 g of polyethylenimine incubated in 24 ml of Opti-MEM at room temperature for 20 min

Lancet 383(9927):15031516 Rossi-Semerano L, Hermeziu B, Fabre M, Kon-Paut I (2011) Macrophage activation syndrome revealing familial Mediterranean fever

difficile , codons for SLP L F274 or SLP H Y27 in pRPF170 were mutated to GCA (Ala) by inverse PCR cloning, yielding plasmids pRPF209 and pJAK186 respectively