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Vascular permeability in ocular disease and the role of tight junctions

Increased GSTP1 expression in tumours and in drug-resistant cells is also the target of a new class of GST suicide inhibitors, including 7-nitro-2,1,3-benzoxadiazole (NBDHEX) (Ricci et al, 2005), which acts to induce apoptosis by promoting dissociation of the GSTP1/JNK1 complex in leukaemia cell lines (Turella et al, 2005)

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The gene-synthesized PSD95-(SH3-GK) (427-724) domain was purified using Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva), followed by thrombin digestion and anion-exchange chromatography (HiTrap Q FF, Cytiva)

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As many post-translational modifications are labile, including phosphorylation, such moieties tend to break first