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Tamura et al., 2000

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Nevertheless, these KO mice fail to respond to mechanical loading in promoting periosteal and endosteal bone formation, and the expression of early mechanoresponsive genes (IGF-I, Cox-2, c-fos), osteogenic markers (RUNX2, and osteocalcin) and canonical Wnt signaling genes (Wnt10b, Lrp5, Dkk1, sFrp2)

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Following the experiment, the femur bones underwent further analysis with mid-diaphyseal peripheral quantitative computed tomography (pQCT) scans

However, de novo protein synthesis is redundant for GH-induced StAR synthesis, suggesting that at least a few testicular actions are IGF-I independent [18]