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The cells were treated with various concentrations of A (1 to 10 M) or 6-OHDA (Tocris, 2547

Ryanodine activation and inhibition of the Ca2+ release channel of sarcoplasmic reticulum

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Thus, the transition from crystal deposition to acute inflammation can be conceptualized as a two-hit process: First hit: MSU crystal deposition on cartilage surfaces activates synovial fibroblasts to release CCL2/MCP-1, recruiting monocytes and establishing subclinical inflammation