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On the other hand, protein restriction reduces nitrogen waste and helps decrease kidney workload by lowering pressure in the capillaries of the kidneys filtration system

The wells were categorized into cell-free, untreated control, untreated control for cell lysis (serving as a control for maximum enzyme activity), and CGA-treated groups

Pfcrt sequencing of the amplicons was done on the 3500 xL ABI Genetic analyzer using version 3.1 of the big dye terminator method (Applied Biosystems)