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Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin

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The results of other researchers 49 indicate that during the life time in the lens some kind of internal lens barrier is developed between nuclear and corticular parts, which hinders the diffusion of molecules to the nucleus

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As a master regulator of immune responses, the nuclear factor-B (NF-B) transcription factor complex governs the expression of numerous inflammation-related genes, including those encoding pro-inflammatory cytokines such as TNF- and interleukin-6 (IL-6), as well as chemokines and adhesion molecules

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