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From a molecular mechanism perspective, RIPK2-mediated signaling activates the NF-B and MAPK pathways, promoting the release of pro-inflammatory cytokines such as TNF-, IL-6, and IL-12/23, thereby driving the inflammatory response

In brief, approximately 1 10 6 cells per group were collected with 0.25% typsin without EDTA and centrifuged at 500 g for 5 min

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The behaviour of the final solution also depends on the peptides purity, salt form, residual solvents, counterions, lyophilisation quality, moisture exposure, storage history, excipients, sequence length, hydrophobicity, and pH profile