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The proteins were extracted from the cells transfected with the pEGFP-USP13, pEGFP-USP13 C345S or pEGFP-C1 plasmids using SDS extraction buffer (100 mM Tris-HCl, pH 8.0, 2% SDS, 1% -mercaptoethanol, 5 mM ethylene glycol tetraacetic acid, 10 mM ethylenediaminetetraacetic acid, 1 M E-64, 1 M bestatin, 1 M pepstatin, 2 M leupeptin, 1 mM phenylmethylsulfonyl fluoride and protease inhibitor cocktail and phosphatase inhibitor)

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Over the past few decades, hyperhomocysteinemia in older adults has become recognized as a modifiable risk factor for cognitive decline, dementia, and Alzheimers disease (64-66)

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