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Cells were incubated in TUNEL reaction mixture (Roche, Germany) at 37 C for one h, washed three times with PBS and incubated in blocking solution for 30 min at room temperature

Finally, to control for antiviral activities of the different MxB variants, we transfected HeLa cells with a similar panel of expression vectors but infected with the VSV-pseudotyped, HIV-1-based luciferase reporter virus NL-Luc

What to Expect During the Test Each testing method has a distinct procedure: Breath Test: A handheld device is used to measure alcohol in exhaled breath