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1d), likely because of its intrinsic lability and some extent of spontaneous unfolding over the 60-min period of the experiment

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Single-cell cloning was performed directly after sorting for GFP + cells in 96-well plates, which were then selected based on the target protein expression and/or sequencing

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On the other hand, in a large cohort of patients with more-advanced T2DM and confirmed coronary artery disease participating in the BARI 2D trial, 50% had confirmed diabetic neuropathy at baseline 17 , and the 4-year cumulative incidence of diabetic neuropathy was 6672% in those without neuropathy at baseline 17

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