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The membrane was washed then probed with the appropriate species-matched secondary horseradish peroxidase conjugated antibody (0.4 mg/mL diluted 1:2500 mouse anti-rabbit IgG (sc-2357, Santa Cruz Biotechnology, Dallas, TX) or 0.4 mg/mL diluted 1:2000 goat anti-mouse IgG (sc-2031)) diluted in 5% skim milk powder in TBST for 1 h at RT on a rocker

Reconstitution technique: When adding bacteriostatic water to the lyophilized powder, direct the stream against the side of the vial and allow it to flow gently onto the powder

Cadmium causes the oxidative modification of proteins in pea plants

doi: 10.1016/j.neuron.2018.06.035 258 NimmerjahnA.KirchhoffF.HelmchenF

If you accidentally bend the needle while preparing your CAVERJECT injection, remove it from the syringe, throw it away, and attach a new, needle to the syringe as described under "Prepare the Dose" below