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[DOI] [PMC free article] [PubMed] [Google Scholar] 297.Zhou Y., Peng Z., Seven E.S., Leblanc R.M

The aphids were maintained under controlled laboratory conditions at 25 2 C, 6070% relative humidity, with an 8-h dark period and a 16-h light period, according to Liu et al

p=0.0002)

Cell transfection The endotoxin-free plasmids were prepared with plasmid miniprep plus purification kit (DP01-Plus-300, GeneMark), purified by ethanol precipitation, and subjected to cell transfection