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When you exercise, you create a small, healthy amount of oxidative stress

doi: 10.1056/NEJMoa060900 50

Doxorubicin (DOX)-induced cardiomyopathy (DIC) A study by Fang et al

For mitochondria analysis, cells were washed twice in RPMI media (no FBS) loaded with 10 nmol ml 1 Mitotracker Green (Thermo Fisher Scientific), 25 nmol ml 1 TMRM (Thermo Fisher Scientific), or 5 mmol ml 1 MitoSox Red for 20 min at 37 C in RPMI (no FBS)