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Proteins were transferred to PVDF membranes and blocked with 5% non-fat milk at room temperature for 1 h

Real-time quantitative reverse transcription PCR The skin tissues from the back of mice were removed and stored at 80C

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Additional studies are needed to assess a possible secondary effect of loss of functional nrf2a in promoting duox /tpo activity dysregulation and the iodination defect leading to hypothyroidism

For example, in DR, TXNIP inhibition paradoxically suppresses autophagy (133), highlighting the tissue-specific and disease context-dependent nature of the autophagic regulation of TXNIP

Int J Mol Sci (2021) 22(20):1095569