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Description

Crypts ( N = 500) per well were seeded in 50 l Matrigel (BD, 356231) on a pre-warmed 24-well plate and allowed to solidify for 10 min at 37 C, after which 500 l IntestiCult Growth Medium supplemented with 100U/ml penicillin and 100 g/ml streptomycin (Biochrome, 0257F) was added

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Hruba et al., 2022) (Figure 1A)

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CFTR is thought to be involved in sperm capacitation and osmoregulation via interactions with ion and water channels

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