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Soluble proteins were extracted from the powdered tissue at 4 C in 1 ml of buffer (pH = 7.5) containing 50 mM HEPES-KOH, 1 mM EDTA, 5 mM DTT, 10% (v/v) glycerol, 2 mM benzamidine, 2 mM aminocuprioc acid, protease inhibitor cocktail mini from Roche Diagnostic

Imaging analyses were performed using Leica Application Suite X (LAS X) software, as described previously 55

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Eur J Cancer 37(Suppl 4):S9-15