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Thank you to our sponsors AG1: Maui Nui: Function: Timestamps (00:00:00) Kyle Gillett (00:00:36) Hormone Health

DSPE-PEG-GSH is conjugated using DSPE-PEG-NHS with activity towards the amine groups in GSH, or GSH is synthesized directly to DSPE-PEG at the N or C-terminal residue

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For ligand preparation, hPD-L1 protein was diluted in 10 mM sodium acetate buffer (pH 4.5) to achieve a working concentration of 5 g/mL, resulting in a final immobilization level of 800 response units (RU) after surface activation