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Figure 2 Transcriptional Regulation of Monocytes Exposed to BDG As the transcriptional regulation GO category was enriched in both up and downregulated genes clusters ( Figure 2E ), we further used the ATAC-seq and RNA-seq datasets to gain insight in the transcriptional regulation of the monocytes response to BDG

Western blotting During immunoblotting, the proteins were extracted with a lysis buffer, and then quantified by means of a bicinchoninic acid (BCA) protein assay kit (Beyotime, Shanghai, China)

Prominin-2 (PROM2) is a glycoprotein physiologically expressed in many normal tissues, mainly in epithelial cells

At the same time, aging also means declining glutathione synthesis, which offsets the negative impact of reactive oxygen species on the bodys cell health and functions

Thats part of why it shows up so frequently in skin biology papers

Patel et al., 2012