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With a eamA deletion and overexpression of P tet -tcyP in a strain with a gmhA deletion there is an increase in the level of the oxidized form of glutathione (GSSG), while additional cysE, cysB and gshAB deletions cause a decrease in the proportion of GSSG in cells E.coli with gmhA (Fig

Finally, the protein was eluted with resuspension buffer (500 mM imidazole without PMSF)

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Multiple comparison post hoc tests were used to determine the statistical difference between different exposure periods and the p-value of less than 0.05 was considered significant

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