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Reconstituted protein should be used immediately or stored in working aliquots at -20C

The formation of gamma-glutamylcysteine is the rate-limiting reaction in GSH synthesis and is feedback inhibited by GSH itself, a mechanism responsible for the regulation of cellular GSH concentration

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This set included proteins previously established as containing hyper-reactive 35,36 or commonly liganded 37 cysteine residues (Supplementary Table 4)

Should you take them together, or at separate times