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eLife 11 , e75055 (2022)

This decline lays the groundwork for the telltale signs of agingwrinkles, diminished mobility, cognitive fog, and flagging energy levels

The following day, plates were washed four times with 1x PBS and 0.02% Tween 20 (ELISA wash buffer) prior to blocking for 1 h with 90 l/well of PBS containing 10% non-fat milk and 0.02% Tween 20 (blocking buffer)