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doi: 10.3390/molecules26134080

If the diluent came from refrigeration, let it reach room temperature first

Rend Lincei Sci Fis E Nat 34:833841

Cells were seeded on 96-well plates at 3 10 4 cells/well in 200l culture media (RPMI 10%FBS, RPMI without cystine and methionine (Merck, # R7513), (RPMI 10% delipidated FBS), Plasmax (CancerTools, #156371) or CSFmax as indicated in figure legend) for 24 h 1S3R-RSL3 (Selleckchem, #S8155), 48 h (Erastin (Selleckchem, # S7242) and Auranofin) and 72 h (iFSP1 (Medchemexpress, #HY-136057)), (SW203668 (Cambridge Biosciences, #GC17354))