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Research shows that overexpression of PER1 sensitizes human cancer cells to the effects of DNA damage-induced apoptosis

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This suggests that, despite the high hydrophobic amino acid content in 7EMSPI and MMSPI, which enhanced emulsifying properties by reducing surface tension (Zhan et al., 2022), the use of methanol and high concentrations of ethanol during defatting led to structural changes in the proteins that increased exposure of hydrophilic groups at pH 9, thus reducing the formation of interfacial membranes

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