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These cells were cultured in RPMI medium (21875-034, Gibco) supplemented with 10% FBS, 1% P/S, and 2 mM L-Glutamine

As mentioned earlier, in order to recover our GST-X protein we have to mix our lysate containg the fusion with glutathione agarose beads

Clinical trials that found a benefit typically measured results after 4 to 12 weeks of daily, uninterrupted use not days

Meanwhile, in this study, we compared the gastrocnemius wet weight ratio among the three groups, and gastrocnemius muscle was used as the selected target to infer the consistent effect of GlyNAC on the involved skeletal muscles