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These data indicated that CRMP5 was not required for the inhibitory effect of NAC on Notch2 and there might be other molecules which worked together with Itch to mediate the degradation of Notch2 by NAC treatment

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Chemiluminescence was monitored using a ChemiDoc-XRS + apparatus (Bio-Rad Laboratories) and quantified using the Image Lab software (version 4.1) Assay for CYP1A activity The relative CYP1A activity was assessed using the P450-Glo CYP1A1 Assay (Promega), with luciferin-CEE as substrate