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30 and 60 min ( Figure 4B )

These changes included consistent increases (transketolase, mitochondrial creatine kinase) and consistent decreases (proteasome subunits alpha type 3, alpha type 6, 26S non-ATPase 14), as well as biphasic patterns in which proteins increased in expression through adulthood but decreased with aging (amphiphysin, dynamin, voltage-dependent anion channel 1)

Using them in tandem yields better outcomes in many cases

Differentiation of myoblasts into myotubes was induced when the cells had achieved 9095% confluence by switching the medium from GM to differentiation medium consisting of DMEM supplemented with 2% horse serum (5 days), then treated as described in the figures

For best results, combine with a diet and exercise program