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To isolate neutrophils, bone marrow cells were subjected to red blood cell lysis and stained with fluorochrome-conjugated anti-mouse antibodies to CD11b (Mac-1, BD Biosciences 553310

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Here, we reviewed overexpressed YAP1 stimulates the production of cytokines, leading to increased infiltration of macrophages, MDSCs, and Tregs, ultimately resulting in an immunosuppressive TME that promotes tumor initiation and progression